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induro reverse transcriptase  (New England Biolabs)


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    Structured Review

    New England Biolabs induro reverse transcriptase
    Induro Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/induro+reverse+transcriptase/Induro+Reverse+Transcriptase/pmc13136890-54-25-29
    Average 98 stars, based on 135 article reviews
    induro reverse transcriptase - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Nonsense-mediated mRNA decay factors target short poly(A)-tailed mRNAs lacking a premature termination codon.
    Article Snippet: .. Reverse transcriptions were performed on resulting 5’P and 5’ cap samples using N6 random primer and Induro reverse transcriptase (NEB) according the manufacturer’s instruction. ..

    Article Title: RNA functional modulation by Mitoxantrone via RNA structural ensemble repartitioning.
    Article Snippet: .. Reactions were then supplemented with 0.5 μl Induro® Reverse Transcriptase (cat. M0681L, New England Biolabs), 0.25 μl SUPERase•InTM RNase Inhibitor, 2 μl 5X RT Buffer [250 mM Tris-HCl pH 8.3; 375 mM KCl; 15 mM MgCl2], and reverse transcription was carried out by incubation at 42°C for 5 min, 55°C for 30 min, 60°C for 30 min and 75°C for 15 min. ..

    Article Title: The lncRNA FENDRR fine-tunes FOXF1 protein levels through a negative feedback loop governing human embryonic lung fibroblast-to-myofibroblast transition
    Article Snippet: RNA integrity was assessed using an Agilent 2100 Bioanalyzer with the RNA 6000 Pico Kit. .. Libraries were prepared from 2 μg of total RNA using the Direct RNA Sequencing Kit (Oxford Nanopore Technologies, SQK-RNA004), including cDNA synthesis with Induro Reverse Transcriptase (NEB, M0681). ..

    Article Title: Inducible, split base editors for in vivo cancer functional genomics.
    Article Snippet: For RNA editing analysis, RNA was extracted from 500,000 cells using an RNeasy Mini kit (74104, Qiagen). .. In total, 0.5–1 μg of RNA was reverse transcribed using Induro Reverse Transcriptase (M0681S, New England Biolabs). .. PCR was performed using the PrimeSTAR GXL Premix system (R051A, Takara).

    Article Title: Systematic assessment of diverse RNA modifications using nanopore direct RNA sequencing
    Article Snippet: .. Reverse transcription was performed by adding a master mix of nuclease-free water, dNTPs (NEB, N0447), and Induro ® RT Reaction Buffer (NEB, B0681AVIAL), followed by Induro ® Reverse Transcriptase (NEB, M0681), and incubated at 60°C for 30 min, then 70°C for 10 min. .. The reverse transcription product was purified with Agencourt RNAClean XP beads (Beckman CoulterTM, A63987) using 70% ethanol washes and eluted in 23 μl nuclease-free water.

    Article Title: HIV-1 infection does not confer intrinsic resistance to cell death induced by cytotoxic T lymphocytes
    Article Snippet: Next, cells were counted and RNA was isolated using the Zymo Quick-RNA viral kit (Cat, # R1034) following the manufacturer protocol, including the Proteinase K and DNase I treatment steps. .. Extracted RNA was quantified using Qubit measurement (Qubit RNA BR reagent kit, ThermoFisher, Cat. # Q10210) and converted into cDNA using oligo-dT and random hexamer primers using Induro Reverse Transcriptase (NEB, Cat. # M0681S) including RNaseOUT (ThermoFisher, Cat. # 10777019). ..

    Article Title: Integrated transcriptomics and proteomics define the TRP channel hierarchy in mouse cortex
    Article Snippet: .. Direct RNA libraries were prepared with the Oxford Nanopore Direct RNA Sequencing Kit (SQK-RNA004) following the manufacturer’s protocol, substituting Induro Reverse Transcriptase (NEB, M0681) for the kit RT and adding 1 μL SUPERase·In during adaptor ligation. ..

    Article Title: Cytoplasmic localization of pseudouridine synthase 7 facilitates a pseudouridine-dependent enhancement of cellular stress tolerance.
    Article Snippet: tRNA was prepared by separating total RNA via anion exchange chromatography on a Resource Q column (Cytiva). .. Reverse transcription to generate the IVT-mRNA library was performed with SuperScript III Reverse Transcriptase (Invitrogen 18080044) for RNA002 and Induro® Reverse Transcriptase (NEB M0681) for RNA004. .. Poly-A RNA enrichment of RNA was performed on total RNA using Dynabeads Oligo(dT) 25 (Thermo, 61005) according to the manufacturer’s recommended protocol.

    Incubation:

    Article Title: RNA functional modulation by Mitoxantrone via RNA structural ensemble repartitioning.
    Article Snippet: .. Reactions were then supplemented with 0.5 μl Induro® Reverse Transcriptase (cat. M0681L, New England Biolabs), 0.25 μl SUPERase•InTM RNase Inhibitor, 2 μl 5X RT Buffer [250 mM Tris-HCl pH 8.3; 375 mM KCl; 15 mM MgCl2], and reverse transcription was carried out by incubation at 42°C for 5 min, 55°C for 30 min, 60°C for 30 min and 75°C for 15 min. ..

    Article Title: Systematic assessment of diverse RNA modifications using nanopore direct RNA sequencing
    Article Snippet: .. Reverse transcription was performed by adding a master mix of nuclease-free water, dNTPs (NEB, N0447), and Induro ® RT Reaction Buffer (NEB, B0681AVIAL), followed by Induro ® Reverse Transcriptase (NEB, M0681), and incubated at 60°C for 30 min, then 70°C for 10 min. .. The reverse transcription product was purified with Agencourt RNAClean XP beads (Beckman CoulterTM, A63987) using 70% ethanol washes and eluted in 23 μl nuclease-free water.

    RNA Sequencing:

    Article Title: The lncRNA FENDRR fine-tunes FOXF1 protein levels through a negative feedback loop governing human embryonic lung fibroblast-to-myofibroblast transition
    Article Snippet: RNA integrity was assessed using an Agilent 2100 Bioanalyzer with the RNA 6000 Pico Kit. .. Libraries were prepared from 2 μg of total RNA using the Direct RNA Sequencing Kit (Oxford Nanopore Technologies, SQK-RNA004), including cDNA synthesis with Induro Reverse Transcriptase (NEB, M0681). ..

    Article Title: Integrated transcriptomics and proteomics define the TRP channel hierarchy in mouse cortex
    Article Snippet: .. Direct RNA libraries were prepared with the Oxford Nanopore Direct RNA Sequencing Kit (SQK-RNA004) following the manufacturer’s protocol, substituting Induro Reverse Transcriptase (NEB, M0681) for the kit RT and adding 1 μL SUPERase·In during adaptor ligation. ..

    cDNA Synthesis:

    Article Title: The lncRNA FENDRR fine-tunes FOXF1 protein levels through a negative feedback loop governing human embryonic lung fibroblast-to-myofibroblast transition
    Article Snippet: RNA integrity was assessed using an Agilent 2100 Bioanalyzer with the RNA 6000 Pico Kit. .. Libraries were prepared from 2 μg of total RNA using the Direct RNA Sequencing Kit (Oxford Nanopore Technologies, SQK-RNA004), including cDNA synthesis with Induro Reverse Transcriptase (NEB, M0681). ..

    Random Hexamer:

    Article Title: HIV-1 infection does not confer intrinsic resistance to cell death induced by cytotoxic T lymphocytes
    Article Snippet: Next, cells were counted and RNA was isolated using the Zymo Quick-RNA viral kit (Cat, # R1034) following the manufacturer protocol, including the Proteinase K and DNase I treatment steps. .. Extracted RNA was quantified using Qubit measurement (Qubit RNA BR reagent kit, ThermoFisher, Cat. # Q10210) and converted into cDNA using oligo-dT and random hexamer primers using Induro Reverse Transcriptase (NEB, Cat. # M0681S) including RNaseOUT (ThermoFisher, Cat. # 10777019). ..

    Ligation:

    Article Title: Integrated transcriptomics and proteomics define the TRP channel hierarchy in mouse cortex
    Article Snippet: .. Direct RNA libraries were prepared with the Oxford Nanopore Direct RNA Sequencing Kit (SQK-RNA004) following the manufacturer’s protocol, substituting Induro Reverse Transcriptase (NEB, M0681) for the kit RT and adding 1 μL SUPERase·In during adaptor ligation. ..



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